kif11 antibody  (Sangon Biotech)

Journal: Journal of translational medicine

Article Title: Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer.

doi: 10.1186/s12967-025-06081-6

Figure Lengend Snippet: Fig. 6 Correlations between the KIF11 expression level and clinical characteristics of patients with EC. A The expression of KIF11 in tumor tissue was significantly greater than that in normal tissue. B The expression of KIF11 in the tumor tissue of patients with EC was significantly greater than that in the corresponding adjacent cancerous tissue. C Differential expression of KIF11 in different histologic grades of EC. D Differential expression of KIF11 in differ ent clinical stages of EC. E Differential expression of KIF11 in different cancer statuses. F Kaplan‒Meier curve for the relationship between KIF11 expression and the prognosis of EC patients

Article Snippet: Next, the membrane was incubated overnight at 4 °C with primary antibodies against KIF11 (1:1000; Catalog # 23333-1-AP; Proteintech, Wuhan, China).

Techniques: Expressing, Quantitative Proteomics

Journal: Journal of translational medicine

Article Title: Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer.

doi: 10.1186/s12967-025-06081-6

Figure Lengend Snippet: Fig. 7 Validation of KIF11 expression in clinical samples. A HE staining of cancer tissues and adjacent tissues from patients with EC. B RT‒qPCR detection of KIF11 expression levels in cancer tissues and adjacent tissues. C Differential expression of KIF11 in cancer and adjacent tissues detected by IHC. Scale bar, 100 μm

Article Snippet: Next, the membrane was incubated overnight at 4 °C with primary antibodies against KIF11 (1:1000; Catalog # 23333-1-AP; Proteintech, Wuhan, China).

Techniques: Biomarker Discovery, Expressing, Staining, Quantitative Proteomics

Journal: Journal of translational medicine

Article Title: Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer.

doi: 10.1186/s12967-025-06081-6

Figure Lengend Snippet: Fig. 8 Effect of KIF11 knockdown on the proliferation of EC cells. The KIF11 knockdown efficiency in the EC cell line ISHIKAWA was determined by RT‒ qPCR (A) and Western blotting (B and C). The KIF11 knockdown efficiency in the EC cell line HEC-1-B was determined by RT‒qPCR (D) and Western blot ting (E and F). G The proliferation of tumor cells after KIF11 knockdown was detected via the MTT assay. **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: Next, the membrane was incubated overnight at 4 °C with primary antibodies against KIF11 (1:1000; Catalog # 23333-1-AP; Proteintech, Wuhan, China).

Techniques: Knockdown, Western Blot, MTT Assay

Journal: Journal of translational medicine

Article Title: Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer.

doi: 10.1186/s12967-025-06081-6

Figure Lengend Snippet: Fig. 9 Effect of KIF11 knockdown on the migration and invasion of EC cell lines. Wound healing assays revealed that KIF11 knockdown reduced the migration ability of ISHIKAWA (A) and HEC-1-B (B) cells 24 h after transfection. Transwell analysis revealed that KIF11 knockdown reduced the invasion of ISHIKAWA (C) and HEC-1-B (D) cells. **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar, 200 μm

Article Snippet: Next, the membrane was incubated overnight at 4 °C with primary antibodies against KIF11 (1:1000; Catalog # 23333-1-AP; Proteintech, Wuhan, China).

Techniques: Knockdown, Migration, Transfection

Journal: Journal of translational medicine

Article Title: Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer.

doi: 10.1186/s12967-025-06081-6

Figure Lengend Snippet: Fig. 10 Effects of KIF11 knockdown on the cell cycle and apoptosis in EC cell lines were examined by flow cytometry. KIF11 knockdown increased the proportion of ISHIKAWA (A) and HEC-1-B (B) cells in the G2/M phase. The increased rate of apoptosis in ISHIKAWA (C) and HEC-1-B (D) cells after KIF11 siRNA transfection. ** p < 0.01, *** p < 0.001

Article Snippet: Next, the membrane was incubated overnight at 4 °C with primary antibodies against KIF11 (1:1000; Catalog # 23333-1-AP; Proteintech, Wuhan, China).

Techniques: Knockdown, Flow Cytometry, Transfection